(Z)-5-(4-methoxybenzylidene)thiazolidine-2,4-dione protects rats from carbon tetrachloride-induced liver injury and fibrogenesis

Figure 3 SKLB010 prevented the nuclear translocation of nuclear factor-κB by inhibiting degradation of IκB. A: The inhibition of nuclear factor-κB (NF-κB)-DNA binding. Carbon tetrachloride (CCl₄)-mediated activation of NF-κB resulted in an increase in DNA-binding activity of NF-κB within 2 h, followed by inactivation of NF-κB at 6 h after stimulation which was demonstrated by a lower DNA-binding activity; B: SKLB010 inhibited the CCl₄-induced degradation of IκB. Total tissue proteins were analyzed by Western blotting at different time points for IκB-α using specific IκB antibodies. β-actin was used as an internal control.