Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array

Figure 2 Vascular endothelial growth factor expression in colorectal cancer cell lines. A: A statistically significant positive correlation is found between vascular endothelial growth factor (VEGF) protein as determined by antibody-array and by enzyme immunoassay (EIA), validating the array method; B and C: Colorectal cancer (CRC) cell lines exhibit variability in VEGF protein (B) and mRNA (C) expression according to their primary or metastatic origin (not statistically significant); D: A statistically significant positive correlation is found between VEGF protein by EIA and VEGF mRNA, suggesting the major role of transcriptional mechanisms in the regulation of VEGF expression. NS: Not significant.