Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array

Figure 1 Angiogenesis-related factors expression profile in colorectal cancer cell lines as determined by cytokine antibody-array. A: K-means (n = 4) clustering grouped the angiogenesis-related proteins according to level of expression; B: Unsupervised-hierarchical clustering of the factors with a significantly different expression in primary and metastatic colorectal cancer (CRC) cell lines; C: Images of subarrays I and II of the primary Caco2 and the metastatic T84 CRC cell lines after detection and processing. IL: Interleukin; MMP: Matrix metalloproteinase; TIMP: Tissue inhibitor of metalloproteinases; GRO: Growth related oncogene; MCP: Macrophage chemoattractant proteins; RANTES: Regulated upon activation normally T-expressed and secreted; uPAR: Urokinase-type plasminogen activator-receptor; G-CSF: Granulocyte colony-stimulating factor; PIGF: Phosphatidylinositol glycan, class F; TNF-α: Tumor necrosis factor-α; GM-CSF: Granulocyte macrophage colony-stimulating factor; IFN-γ: Interferon γ; IGF: Insulin-like growth factor; PECAM: Platelet-endothelial cell adhesion molecule; I-TAC: Inducible T cell α chemoattractant protein; ENA: Epithelial neutrophil activating protein; EGF: Epidermal growth factor; PDGF-BB: Platelet-derived growth factor, β polypeptide; TGF-β1: Transforming growth factor β1; Neg: Negative control; Pos: Positive control.