Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array

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Feb 21, 2012 (publication date) through Dec 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 21, 2012; 18(7): 637-645
Published online Feb 21, 2012. doi: 10.3748/wjg.v18.i7.637

Table 2 Primer and probe sequences for vascular endothelial growth factor isoforms quantitative reverse-transcription polymerase chain reaction

	Sense primer	Antisense primer	Taqman probe	Amplicon size (bp)
VEGF end-point and cloning	ACTGCCATCCAATCGAGACC	GATGGCTTGAAGATGTACTCGATCT
GAPDH end-point and cloning	TGGTATCGTGGAAGGACTCATGAC	ATGCCAGTGAGCTTCCCGTTCAGC		189
VEGF₁₂₁ mRNA	CAAGGCCAGCACATAGGAGA	CTCGGCTTGTCACATTTTTC	CTTCCTACAGCACAACAAATGTGAATGCAGA	101
VEGF₁₆₅ mRNA	TGTGAATGCAGACCAAAGAAAGA	TGCTTTCTCCGCTCTGAGC	AGAGCAAGACAAGAAAATCCCTGTGGGC	74
VEGF₁₈₉ mRNA	CGCAAGAAATCCCGGTATAAGT	TGCTTTCTCCGCTCTGAGC	AGGCCCACAGGGAACGCTCCAG	65
GAPDH	TGGTATCGTGGAAGGACTCATGAC	ATGCCAGTGAGCTTCCCGTTCAGC	CCCAGAGACTGTGGATGGCCCC	189

VEGF: Vascular endothelial growth factor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Citation: Abajo A, Bitarte N, Zarate R, Boni V, Lopez I, Gonzalez-Huarriz M, Rodriguez J, Bandres E, Garcia-Foncillas J. Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array. World J Gastroenterol 2012; 18(7): 637-645
URL: https://www.wjgnet.com/1007-9327/full/v18/i7/637.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i7.637