Epigenetic inactivation of secreted frizzled-related protein 2 in esophageal squamous cell carcinoma

Figure 1 Expression and promoter methylation of secreted frizzled-related protein 2 in esophageal squamous cell carcinoma cell lines. A: Secreted frizzled-related protein 2 (SFRP2) mRNA expression as determined by reverse-transcription polymerase chain reaction (RT-PCR); B: The methylation status of the SFRP2 promoter as determined by methylation specific PCR; C: mRNA expression of SFRP2 (determined by RT-PCR) restored after treatment with the demethylation agent 5-Aza-dC in three esophageal squamous cell carcinoma cell lines. M: Methylated; U: Unmethylated.