Epigenetic inactivation of secreted frizzled-related protein 2 in esophageal squamous cell carcinoma

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Feb 14, 2012 (publication date) through Jul 31, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 14, 2012; 18(6): 532-540
Published online Feb 14, 2012. doi: 10.3748/wjg.v18.i6.532

Table 1 List of primer sequences

Primer	Forward primer (5'-3')	Reverse primer(5'-3')
RT-PCR
SFRP2	5’-GATGATGACAACGACATAATGGAAACG -3’	5’-GAGTGTGCTTGGGGAACGGGAGCT-3’
GAPDH	5’-CCCTTCATTGACCTCAACTACATGG-3’	5’-CATGGTGGTGAAGACGCCAG-3’
SFRP2(CDS)	5’-CCAAGCTTATGCTGCAGGGCCCTGGCTCGC-3’	5’-CGGAATTCCTAGCACTGCAGCTTGCGGATGCTG-3’
MSP
SFRP2-M	5’-GGGTCGGAGTTTTTCGGAGTTGCGC-3’	5’-CCGCTCTCTTCGCTAAATACGACTCG-3’
SFRP2-U	5’-TTTTGGGTTGGAGTTTTTTGGAGTTGTGT-3’	5’-AACCCACTCTCTTCACTAAATACAACTCA-3’
BSP
SFRP2	5’-AAAAAGGTTAAGAAAATTTTGGT-3’	5’-AACCAAAACCCTACAACATC-3’

SFRP2: Secreted frizzled-related protein 2; GAPDH: Glyceraldehyde-3-phosohate dehydrogenase; MSP: Methylation-specific.

Citation: Hao XW, Zhu ST, He YL, Li P, Wang YJ, Zhang ST. Epigenetic inactivation of secreted frizzled-related protein 2 in esophageal squamous cell carcinoma. World J Gastroenterol 2012; 18(6): 532-540
URL: https://www.wjgnet.com/1007-9327/full/v18/i6/532.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i6.532