A pharmacodynamic model of portal hypertension in isolated perfused rat liver

Table 3 Acetylcholine reduces portal pressure (mean ± SE, n_i = 4)

Log[Ach (mol/L)]	d0	d28	d56	d84
-13	-0.16 ± 0.12	-0.002 ± 0.01	-0.076 ± 0.08	-0.001 ± 0.01
-12	-0.19 ± 0.10	-0.009 ± 0.01	-0.091 ± 0.10	-0.006 ± 0.01
-11	-0.31 ± 0.07	-0.025 ± 0.01	-0.125 ± 0.15	-0.012 ± 0.01
-10	-0.39 ± 0.08	-0.035 ± 0.18	-0.176 ± 0.23	-0.019 ± 0.01
-9	-0.42 ± 0.08	-0.043 ± 0.04	-0.203 ± 0.26	-0.041 ± 0.02
-8	-0.44 ± 0.12	-0.049 ± 0.05	-0.225 ± 0.26	-0.050 ± 0.03
-7	-0.47 ± 0.14	-0.052 ± 0.07	-0.256 ± 0.28	-0.054 ± 0.03

Primary data were used to calculate the dose-effect relationship between acetylcholine and portal vein relaxation in the isolated portal perfused rat livers without chronic hepatitis, where rats were administered 3 mL/kg olive oil subcutaneously twice per week for 84 d (control rats) (d₀). Rats with chronic hepatitis at three stages, hepatic degeneration (d₂₈), fibrosis (d₅₆) and cirrhosis (d₈₄), were administered 3 mL/kg 40% (v/v) CCl₄ in olive oil subcutaneously twice per week for 28 d, 56 d and 84 d, respectively. Ach: Acetylcholine.