A pharmacodynamic model of portal hypertension in isolated perfused rat liver

Table 1 Hepatic pathological changes in model rats (mean ± SE, n_i = 8)

Advanced	Lobule ratio	Collagen ratio
d0	0.38 ± 0.05	0.0000700 ± 0.0001180
d28	0.33 ± 0.04b	0.0019658 ± 0.0024864b
d56	0.11 ± 0.04b,d	0.0043315 ± 0.0048768b,d
d84	0.06 ± 0.01b,d,f	0.0143996 ± 0.0143860b,d,f

Lobule ratio is the average percentage obtained from ten random fields using Image-Pro Plus v 5.1 software, and each field had a ratio of lobule area per total analyzed field area in hematoxylin-eosin stained sections under a Digital Pathology System at × 20 magnification from isolated portal perfused rat livers. Collagen ratio is the average percentage obtained from ten random fields using Image-Pro Plus v 5.1 software, and each field had a ratio of collagen area per total analyzed field area in Masson-stained sections under a Digital Pathology System at × 40 magnification from isolated portal perfused rat livers. a and b denote significant (^bP ＜ 0.01) differences between those at d₀ and at d₂₈, d₅₆, or d₈₄. c and d denote significant (^cP ＜ 0.05 and ^dP ＜ 0.01) differences at d₂₈ and at d₅₆, or d₈₄. e and f denote significant (^eP ＜ 0.05 and ^fP ＜ 0.01) differences at d₅₆ and at d₈₄.