Impact of intestinal ischemia/reperfusion and lymph drainage on distant organs in rats

Figure 3 Expression levels of high mobility group protein box 1 in the liver, lung, and kidney (n = 8). Top: Western blotting analysis of high mobility group protein box 1 (HMGB1) in the liver (left), lung (middle), and kidney (right). Lane 1: Blank; Lane 2: Sham; Lane 3: I/R + D; Lane 4: I/R; Bottom: Quantification of HMGB1 protein levels in the liver (left), lung (middle), and kidney (right). HMGB1 levels were normalized to the levels of the β-actin internal control. ^aP < 0.05 vs the blank group; ^cP < 0.05 vs the sham group; ^eP < 0.05 vs the I/R group. I/R + D: Ischemia/reperfusion and drainage; I/R: Ischemia/reperfusion.