Mechanisms of cholecystokinin-induced calcium mobilization in gastric antral interstitial cells of Cajal

Figure 4 Sulfated cholecystokinin-8 induced calcium mobilization in cultured interstitial cells of Cajal. A, B: Pretreatment with 5 μmol/L thapsigargin (A) or 1 μmol/L xestospongin C (B) completely abolished sulfated cholecystokinin-8 (CCK-8S)-induced [Ca²⁺]i increases; C: Addition of nifedipine resulted in a smaller peak of [Ca²⁺]i in comparison with normal conditions; D: The CCK-8S-elicited [Ca²⁺]i increase in the calcium-free medium was lower than that in the calcium-containing buffer; E: Quantification of [Ca²⁺]i changes following addition of nifedipine or removal of extracellular Ca²⁺. The fluorescence was normalized as F1/F0 (F1: Maximal fluorescence after drug addition; F0: Basal fluorescence before drug addition). ^bP < 0.01 vs control.