Mechanisms of cholecystokinin-induced calcium mobilization in gastric antral interstitial cells of Cajal

Figure 2 The regulation of sulfated cholecystokinin-8 on [Ca²⁺]i in cultured interstitial cells of Cajal from the murine gastric antrum. A1: Fluorescent intensity image of Fluo-3/AM loaded cultured interstitial cells of Cajal (ICC) under normal conditions; A2-6: Fluorescent intensity gradually increased in the presence of cholecystokinin-8 (CCK-8S) (100 nmol/L); B-E: Effects of different concentrations of sulfated CCK-8S on mean [Ca²⁺]i. In each case, cells from at least five different cell cultures; F: Effects of CCK-8S were estimated as percentage of ΔF/F0, where F0 was derived from the averaged intensity of the first 10-30 frames minus the background in the cell-free region and ΔF is fluorescent intensity of the response minus F0. ^aP < 0.05, ^bP < 0.01 vs control.