Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells

Figure 3 Gardenia jasminoides significantly reduced the expression of fibrotic marker genes in a human hepatic stellate cells line. Cells were exposed to transforming growth factor-β1 (TGF-β1) (5 ng/mL) in combination with the indicated concentrations of Gardenia jasminoides or vehicle for 24 h. A: mRNA expression of TGF-β1 in LX-2 cells; B: mRNA expression of collagen type I(Col I)  in LX-2 cells; C: Western blotting analysis of α-smooth muscle actin (α-SMA) expression in LX-2 cells. ^bP < 0.01 vs sham; ^cP < 0.05, ^dP < 0.01 vs TGF-β1 + vehicle (n = 3).