Diagnosis of intestinal tuberculosis using a monoclonal antibody to Mycobacterium tuberculosis

Figure 2 Histopathological views of tuberculous granuloma and localization of the mycobacterial antigen in the colonic lesion (patient 8). A: A granuloma, surrounded by inflammatory lymphocytes, is present in the lamina propria (hematoxylin and eosin, × 200); B: Immunohistochemical staining view of the colonic specimen (× 200). Note that the mycobacterial antigens (brownish granular matter) are present in the cytoplasm of epithelioid histiocytes and multinucleated giant cells in the granuloma; C: Cells stained with the pan-macrophage marker CD68 antibody are present in the granuloma (× 400); D: The mycobacterial antigens (brownish granular matter) are present in the cytoplasm of the macrophages in the granuloma (× 400).