Esophageal tissue engineering: A new approach for esophageal replacement

Figure 2 Production of esophageal natural acellular matrices. Decellularization involves treatment of fresh esophageal tissue with a combination of solutions that will remove the cells but maintain the structural characteristics of the native extracellular matrix. The optimal methodology of esophageal decellularization is currently under investigation. Our experience with the detergent enzymatic treatment is illustrated here with hematoxylin and eosin staining of a representative decellularized esophagus demonstrating preservation of the native architecture (Scale bar 100 μm).