Associations between interleukin-1 polymorphisms and gastric cancers among three ethnicities

doi:10.3748/wjg.v18.i47.7093

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 21, 2012; 18(47): 7093-7099
Published online Dec 21, 2012. doi: 10.3748/wjg.v18.i47.7093

Table 1 Primer sequences, polymerase chain reaction and denaturing high-performance liquid chromatography conditions for detection of gene polymorphisms

Gene	Primer sequence	PCR annealing temperature (°C)	PCR product size (bp)	DHPLC application type	Oven temperature (°C)
IL-1B-31	F: AGAAGCTTCCACCAATACTC	60	240	Mutation	59
IL-1B-31	R: AGCACCTAGTTGTAAGGAAG	60	240	Mutation	59
IL-1B-511	F: TGGCATTGATCTGGTTCATC	58.5	306	Mutation	60.5
IL-1B-511	R: GTTTAGGAATCTTCCCACTT	58.5	306	Mutation	60.5
IL-1 RN	F: CCCCTCGAGCAACATCC	59	270-442	VNTR	50.0
IL-1 RN	R: GGTCAGAAGGGCAGAGA	59	270-442	VNTR	50.0

DHPLC: Denaturing high-performance liquid chromatography; VNTR: Variable number of identical tandem repeats; PCR: Polymerase chain reaction; IL: Interleukin.

Citation: Zhao JD, Geng PL, Li ZQ, Cui S, Zhao JH, Wang LJ, Li JZ, Ji FX, Li GY, Shen GS, Lin MZ, Shen CF, Cao CZ. Associations between interleukin-1 polymorphisms and gastric cancers among three ethnicities. World J Gastroenterol 2012; 18(47): 7093-7099
URL: https://www.wjgnet.com/1007-9327/full/v18/i47/7093.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i47.7093