Hepatoprotective effects of baicalein against CCl4-induced acute liver injury in mice

Figure 5 The microRNA levels of tumor necrosis factor-α, interleukin-6, transforming growth factor-α, hepatocyte growth factor and epidermal growth factor in liver of control and baicalein groups after carbon tetrachloride (1 mL/kg) treatment. Total RNA was isolated from liver tissue using TRIzol methods and quantified spectrophotometrically at 260 nm. The mRNA levels of tumor necrosis factor-α (TNF-α) (A), interleukin-6 (IL-6) (B), hepatocyte growth factor (HGF) (C), epidermal growth factor (EGF) (D) and transforming growth factor-α (TGF-α) (E) genes were quantified using reverse transcription polymerase chain reaction and normalized to β-actin housekeeping gene. Values represent mean ± SE (n = 6). P < 0.05, P < 0.01 between control and baicalein groups.