Clinical significance of human kallikrein 12 gene expression in gastric cancer

Figure 5 Efficiency of small interfering RNA in silencing the kallikrein 12 mRNA, and protein expression in MKN-45 cells. A: MKN-45 cells transfected with small interfering RNA targeting human kallikrein 12 (KLK12) gene showed a remarkable decrease in the level of KLK12 mRNA compared to mock-transfected or parent MKN-45 cells. Data are shown as mean ± SD, using the one-way analysis of variance test (^bP < 0.01 vs other cell lines); B: Western blotting analysis showed a reduced protein expression in KLK12 small interfering RNA (siRNA) transfected cells. The protein levels are measured by Image J software (National Institutes of Health, United States) with β-actin protein normalization.