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World J Gastroenterol. Nov 28, 2012; 18(44): 6420-6426
Published online Nov 28, 2012. doi: 10.3748/wjg.v18.i44.6420
Published online Nov 28, 2012. doi: 10.3748/wjg.v18.i44.6420
Figure 1 rAd-SP-rINSfur administration reduces high blood glucose levels to normal physiological range in Leprdb/db mice.
A: Schematic diagram of adenoviral construct expressing furin cleavable insulin gene under the control of synthetic promoter. The synthetic promoter is composed of nine transcription factor binding elements upstream of pyruvate kinase basal promoter (L-PK). A furin-cleavable site was introduced at the junction of the B-chain and C-peptide; B: Blood glucose levels; C: Body weights of Leprdb/db mice treated with rAd-CMV-βgal or rAd-SP-rINSfur. Viruses were injected into experimental animals at 12 wk of age with a dose of 3 × 1010 viral particles, and blood glucose levels and body weights were monitored. Blood glucose levels and body weights were measured between 2-4 pm during the day, and food and water were given ad libitum during the experiments. All values are mean ± SE. Day 0 indicates the day of viral injection. HNF-1: Hepatocyte nuclear factor-1; C/EBP: CCAAT enhancer binding protein; GlRE: Glucose responsive elements; HNF-4: Hepatocyte nuclear factor-4.
- Citation: Han J, Kim EH, Choi W, Jun HS. Glucose-responsive artificial promoter-mediated insulin gene transfer improves glucose control in diabetic mice. World J Gastroenterol 2012; 18(44): 6420-6426
- URL: https://www.wjgnet.com/1007-9327/full/v18/i44/6420.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i44.6420