Exogenous carbon monoxide attenuates inflammatory responses in the small intestine of septic mice

Figure 5 Effects of tricarbonyldichlororuthenium (II) dimer carbon monoxide-releasing molecules on protein expression of intercellular adhesion molecule 1 and inducible nitric oxide synthase in the mid-ileum of cecal ligation and perforation-induced mice. A: Representative experiment; B and C: Quantitative results (average optical density, A/mm²) of three. Mice were challenged with cecal ligation and perforation (CLP) and treated with tricarbonyldichlororuthe-nium (II) dimer. Protein expression of intercellular adhesion molecule 1 (ICAM-1) and inducible nitric oxide synthase (iNOS) in the mid-ileum was determined by Western blotting at 24 h after CLP. Lane 1: Sham group; Lane 2: CLP group; Lane 3: CLP + carbon monoxide-releasing molecule (CORM)-2 group; Lane 4: CLP + inactivated-carbon monoxide-releasing molecule (iCORM)-2 group. ^bP < 0.01 vs sham group; ^cP < 0.05 vs CLP group.