Ethyl pyruvate protects against experimental acute-on-chronic liver failure in rats

Figure 3 Effect of ethyl pyruvate on high mobility group box-1 expression in liver tissues of rat acute-on-chronic liver failure model. A: A representative reverse transcription-polymerase chain reaction specific for high mobility group box-1 (HMGB1) mRNA and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA (internal control) in each group shown in the electrophotogram; B: Western blot assay showing the expression of HMGB1 protein in the liver tissues of rats in each group. ^bP < 0.01 vs normal; ^cP < 0.05 vs model; ^eP < 0.05 vs ethyl pyruvate (EP) treatment at 3 h. The time points of 3 h, 6 h, 12 h and 24 h represent four subgroups of EP treatment group. Results are expressed as mean ± SD (n = 5). N: Normal group; M: Model group.