Effects and mechanisms of store-operated calcium channel blockade on hepatic ischemia-reperfusion injury in rats

Figure 6 Effects of store-operated calcium channel blockers on cell survival or injuries of normal and hepatic ischemia-reperfusion injuried hepatocytes. A: MTT assay of normal hepatocytes alone or co-cultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB, or 100 μmol/L La³⁺ (n = 4), the cell survival rate is not significantly different (P > 0.05); B: MTT assay of HIRI hepatocytes alone or cocultivated with 0.025% (v/v) DMSO, 100 μmol/L 2-APB or 100 μmol/L La³⁺ (n = 4), the average optical absorbance value in hepatocytes pretreated with 100 μmol/L 2-APB (0.79 ± 0.05) was higher than in untreated hepatocytes (0.69 ± 0.04), ^aP = 0.027; C: Compared with normal hepatocytes, HIRI hepatocytes incurred cell damage (n = 4), ^aP = 0.029. HIRI: Hepatic ischemia-reperfusion injury; 2-APB: 2-aminoethoxydiphenyl borate; DMSO: Dimethylsulfoxide; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.