Effects and mechanisms of store-operated calcium channel blockade on hepatic ischemia-reperfusion injury in rats

Figure 1 Agonist-induced Ca²⁺ oscillations are inhibited by blocking Ca²⁺ entry in freshly isolated rat hepatocytes. Traces show Ca²⁺ oscillations in freshly isolated rat hepatocytes, with the abscissa axis as time (min), the vertical axis as Fluo-4 fluorescence intensity (∆F/F0), which demonstrates the changes of [Ca²⁺]_i. The number of hepatocytes measured is indicated by “n”. A: The fluorescence image (left) and microscopic image (right) of freshly isolated hepatocytes loaded with fluo-4/acetoxymethyl. Ca²⁺ oscillations were initiated by 100 nmol/L noradrenaline in the presence (B, n = 27) or absence (C, n = 16) of extracellular Ca²⁺. Ca²⁺ oscillations induced by 0.5 nmol/L adenosine-triphosphate were inhibited by 20 μmol/L 2-APB in the presence (D, n = 14) or absence (E, n = 11) of extracellular Ca²⁺. 2-APB: 2-aminoethoxydiphenyl borate.