Adenovirus-expressed preS2 antibody inhibits hepatitis B virus infection and hepatic carcinogenesis

Figure 1 Expression and identification of the Ad315-preS2Ab-mediated preS2Ab. A: ELISA results show that the expression levels of the preS2Ab increase in the supernatants of Ad315-preS2Ab-infected L02 cells with increases in MOI; B: Western blotting shows that the expression of preS2Ab is high at an MOI of 100 pfu/cell. GAPDH is used as the loading control; C: The expressed preS2Ab in the supernatant of Ad315-preS2Ab-infected L02 cells recognizes liver cells from HBV transgenic Imprinting Control Region (ICR) mice as shown by a strong immunofluorescent reaction (left). Liver cells of normal ICR mice do not show labeling (right) (× 200). ELISA: Enzyme linked immunosorbent assay; HBV: Hepatitis B virus; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MOI: Multiplicity of infection.