Identification of deregulated miRNAs and their targets in hepatitis B virus-associated hepatocellular carcinoma

Figure 4 Cyclin D3 and clathrin heavy chain are the direct target of miR-138 and miR-199a-5p. A: Abundance of deregulated miRNAs in hepatitis B virus (HBV)-hepatocellular carcinoma (HCC) non-tumor tissues; B: The putative miR-138 or miR-199a-5p binding sequence in the 3’-UTR of cyclin D3 (CCND3) or clathrin heavy chain (CHC) mRNA; C: The expression of CCND3 and CHC in 4 paired HCCs (T) and adjacent non-tumor tissues (N); D: Suppressed luciferase activity of wild type 3’UTR of CCND3 or CHC by miR-138 or miR-199a-5p mimic. Firefly luciferase activity of each sample was mea sured 48 h after transfection and normalized to Renilla luciferase activity; E: Suppressed expression of endogenous CCND3 or CHC in HepG2 cells by miR-138 or miR-199a-5p mimic, respectively. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. Column, mean of three independent experiments; bars, SD; ^aP < 0.01 vs control group. NC: Non-relative control.