Protection of ghrelin postconditioning on hypoxia/reoxygenation in gastric epithelial cells

Figure 5 Effects of D-Lys3-GHRP-6, capsazepine and LY294002 in ghrelin postconditioning on the expression of Bcl-2, Bax, Akt and glycogen synthase kinase-3β in human gastric epithelial cells induced by hypoxia/reoxygenation. Cells were grouped as follows: normoxic culture for 6 h (N), 2 h hypoxia/4 h reoxygenation (H/R), DMSO vehicle postconditioning (DM), ghrelin postconditioning (10^-7 mol/L) (GH) D-Lys3-GHRP-6 + ghrelin postconditioning (D + GH), capsazepine + ghrelin postconditioning (C + GH) and LY294002 + ghrelin postconditioning (L + GH). The expression of β-actin was detected as an internal standard. Densitometry results are expressed as ratio of test over normal group. A, B: Bcl-2 expression; C, D: Bax expression; E: Akt expression; F: Glycogen synthase kinase (GSK)-3β expression. mean ± SD. n = 6. ^bP < 0.01 vs H/R, ^dP < 0.01 vs GH.