Axl glycosylation mediates tumor cell proliferation, invasion and lymphatic metastasis in murine hepatocellular carcinoma

Figure 3 Axl deglycosylation alters the invasive ability of Hca-F cells in vitro. In vitro ECMatrix gel analysis. Wright-Giemsa staining results of the lower surface filter showed that the cells passed through the filter and attached to the lower side (400 ×). The average number of cells invading the filter was counted. Cells treated with tunicamycin (A) or PNGase F (B) were significantly less invasive (^aP < 0.05 vs untreated Hca-F cells) than untreated Hca-F cells. Data was obtained in triplicate.