Axl glycosylation mediates tumor cell proliferation, invasion and lymphatic metastasis in murine hepatocellular carcinoma

Figure 2 Axl deglycosylation effects on cell proliferation in vitro. Hca-F cells were exposed to tunicamycin or PNGase F and harvested at 24 h, 48 h, 72 h, 96 h, and 120 h. Cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Decreased proliferative ability was detected in cells treated with tunicamycin (A) or PNGase F (B), compared with untreated Hca-F cells. Data was obtained in triplicate.