Inhalation of hydrogen gas reduces liver injury during major hepatotectomy in swine

Figure 6 Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining after hepatectomy and protective effect of H₂ against liver apoptotic cell death in two groups. A: Revealed many terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells (arrows, identified morphologically by dark brown staining nuclei) in the liver remnant × 400; B: Microphotometric evaluation of apoptotic index (AI) in TUNEL-stained tissue after hepatotectomy for 3 h. A bar graph shows the mean ± SD of AI level (%) in two groups. Each group is represented by the mean of 7 swines. In hydrogen gas treated-group, the AI level was significantly low to Contrast-group (^aP < 0.05 vs Contrast-group).