Tumor necrosis factor alpha increases intestinal permeability in mice with fulminant hepatic failure

Figure 4 Mucosal colonic tissues from fulminant hepatic failure and control mice were analyzed for the protein expression of occludin and claudin-1 by Western blotting analysis. A: Occludin expression decreased significantly 6, 9 and 12 h after D-galactosamine (GalN)/lipopolysaccharide (LPS) injection; B: Occludin expression decreased significantly 6 h after injection in the GalN/ tumor necrosis factor alpha (TNF-α)- and TNF-α-treated mice; C: Claudin-1 expression decreased significantly 6 h and 9 h after GalN/LPS injection; D: Claudin-1 expression decreased significantly 6 h after injection in the GalN/TNF-α- and TNF-α-treated mice. NS: Normal saline; FHF: Fulminant hepatic failure. ^aP < 0.05, ^bP < 0.01 vs baseline. Data are reported as the mean ± SD.