Interplay of neuropilin-1 and semaphorin 3A after partial hepatectomy in rats

Figure 4 Role of semaphorin 3A in sinusoidal endothelial cell migration and apoptosis. A: Effect of semaphorin 3A (Sema3A) on migration of sinusoidal endothelial cells (SECs) isolated from normal rats (CONT-SECs) and rats after partial hepatectomy (PHx-SECs). SEC migration was assessed by cell transwell assay in the presence or absence of 5 nmol Sema3A for 24 h. Migration index represents the number of migratory cells/number of migratory cells in vehicle-treated PHx-SECs; B: Effect of Sema3A on apoptosis of CONT-SECs and PHx-SECs. Following incubation of SECs with 5 nmol Sema3A or vehicle for 24 h, apoptotic cells were determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling method. Results are expressed as the mean ± SE. ^aP < 0.05, ^bP < 0.01 vs vehicle-treated PHx-SECs (n = 4).