Copyright
©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Sep 28, 2012; 18(36): 5129-5134
Published online Sep 28, 2012. doi: 10.3748/wjg.v18.i36.5129
Published online Sep 28, 2012. doi: 10.3748/wjg.v18.i36.5129
Table 1 Primer sets for nested methylation-specific polymerase chain reaction
| Gene | Primer sequence (5'→3') | ||
| APC | U | F | GTGTTTTATTGTGGAGTGTGGGTT |
| R | CCAATCAACAAACTCCCAACAA | ||
| M | F | TATTGCGGAGTGCGGGTC | |
| R | TCGACGAACTCCCGACGA | ||
| WIF-1 | U | F | GGGTGTTTTATTGGGTGTATTGT |
| R | AAAAAAACTAACACAAACAAAATACAAAC | ||
| M | F | CGTTTTATTGGGCGTATCGT | |
| R | ACTAACGCGAACGAAATACGA | ||
| RUNX-3 | U | F | TTATGAGGGGTGGTTGTATGTGGG |
| R | AAAACAACCAACACAAACACCTCC | ||
| M | F | TTACGAGGGGCGGTCGTACGCGGG | |
| R | AAAACGACCGACGCGAACGCCTCC | ||
| DLC-1 | U | F | AAACCCAACAAAAAAACCCAACTAACA |
| R | TTTTTTAAAGATTGAAATGAGGGAGTG | ||
| M | F | CCCAACGAAAAAACCCGACTAACG | |
| R | TTTAAAGATCGAAACGAGCGAGCG | ||
| SFRP-1 | U | F | GAGTTAGTGTTGTGTGTTTGTTGTTTTGT |
| R | CCCAACATTACCCAACTCCACAACCA | ||
| M | F | GTGTCGCGCGTTCGTCGTTTCGC | |
| R | AACGTTACCCGACTCCGCGACCG | ||
| DKK | U | F | TTAGGGGTGGGTGGTGGGGT |
| R | CTACATCTCCACTCTACACCCA | ||
| M | F | GGGGCGGGCGGCGGGGC | |
| R | ACATCTCCGCTCTACGCCCG | ||
| E-cad | U | F | TAATTTTAGGTTAGAGGGTTATTGT |
| R | CCACCCCAATACTAAATCACAACA | ||
| M | F | TTAGGTTAGAGGGTTATCGCGT | |
| R | TAACTAAAAATTCACCTACCGAC |
-
Citation: Liu JB, Wu XM, Cai J, Zhang JY, Zhang JL, Zhou SH, Shi MX, Qiang FL. CpG island methylator phenotype and
Helicobacter pylori infection associated with gastric cancer. World J Gastroenterol 2012; 18(36): 5129-5134 - URL: https://www.wjgnet.com/1007-9327/full/v18/i36/5129.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i36.5129