Coexistence of hyperlipidemia and acute cerebral ischemia/reperfusion induces severe liver damage in a rat model

Figure 2 Effect of hyperlipemia plus ischemia/reperfusion on the activity and expression of CYP2E1 in the liver of rats after high-fat diet treatment for 18 wk and/or 2 h transient focal cerebral ischemia followed by 24 h reperfusion. A: Hepatocytes in the hyperlipemia (HL) + ischemia/reperfusion (I/R) group showed increased cytochrome P450 2E1 (CYP2E1) expression examined by immunohistochemical staining (× 200) than those in other groups. Slides are representative of 6-10 animals per group; B: Microsomal hydroxylase activity of the CYP2E1-containing enzyme measured with aniline as the substrate was much higher in the HL+I/R group; C: HL+I/R enhanced the expression level of CYP2E1 protein in liver microsomes, which was confirmed by Western blotting. Values are the mean ± SD. ^aP < 0.05, ^bP < 0.01 vs control group; ^dP < 0.01 vs HL group; ^fP < 0.01 vs I/R group.