Indomethacin but not a selective cyclooxygenase-2 inhibitor inhibits esophageal adenocarcinogenesis in rats

Figure 2 Pathological findings in the esophagus of rats with esophagojejunostomy. A: Hyperplasia of squamous epithelium in a control rat esophagus; B: Hematoxylin and eosin (HE) staining (× 100) showing an area of intestinal metaplasia without dysplasia. The top picture shows in more detail a gland (× 400); C: Indomethacin-treated rat. HE staining (× 40) of an area of intestinal metaplasia showing a focus with low-grade dysplasia (black arrow). The top pictures show in more detail the area with low grade dysplasia (× 200; × 400). Glands conserve their architecture. Nuclear pseudostratification can be seen, nuclear size is also increased but nuclei do not reach the lumen; D: HE staining of an area of intestinal metaplasia showing high grade dysplasia. Magnification × 100; the top picture illustrates at higher magnification (× 400), some glands with high grade dysplasia, characterized by loss of nuclear polarity and atypia; E: Photomicrograph shows an adenocarcinoma developed inside the intestinal metaplasia of the esophagus near the jejunum epithelium in a rat that had undergone an esophagojejunostomy. HE staining shows that the esophageal adenocarcinoma consists of dilated, cystic glands with abundant mucin secretion and epithelial dysplasia. (Magnification × 40; the top picture shows the same fields at × 100 magnification).