Gastro protective properties of the novel prostone SPI-8811 against acid-injured porcine mucosa

Figure 3 Expression of tight junction proteins in membrane fractions in acid injured porcine gastric mucosa. A: Western analysis revealed expression of occludin in the detergent insoluble in tissues treated with normal Ringer’s (NR) alone, with very little discernable occludin in the detergent soluble fraction. On the other hand, treatment with acid (pH of 1.5 for 90 min) markedly reduced the expression of occludin in the detergent insoluble fraction, with a similar lack of occludin expression in the detergent soluble fraction. Pretreatment of tissues with mucosal SPI-8811 resulted in expression of occludin in the different fractions to a very similar extent as control tissues; B: Western analysis revealed expression of whole occludin in either control, acid injured and SPI-8811 pretreated and acid injured porcine gastric mucosa from Ussing chambers. Acid injury markedly reduced expression of whole occludin compared to control while pretreatment with SPI-8811 preserved the loss of occludin from acid injury. DS: Detergent soluble; DIS: Detergent insoluble.