Gastro protective properties of the novel prostone SPI-8811 against acid-injured porcine mucosa

Figure 2 Histological findings in acid-injured gastric mucosal tissues. A: Tissues bathed in normal Ringer’s appeared uninjured after 90 min in Ussing chambers, whereas those exposed to acid had substantial evidence of epithelial injury (× 100). Pretreatment with SPI-8811 ameliorated injury evident in acid-injured tissues. Each panel is representative of at least 3 separate animals; B: Periodic acid-schiff stain (PAS) staining findings in control tissues bathed in Ringer’s solution for 90 min appeared to have normal expression of surface mucus by PAS-alcian blue staining whereas tissues exposed to acid had distinct loss to surface mucus staining (× 100). However, pretreatment with SPI-8811 prevented the loss of mucus in response to acid and thus preserved the surface mucus; C: Immunofluorescence findings for tight junction protein occludin reveals control tissues bathed in Ringer’s solution for 90 min appeared to have normal immunolocalization of the tight junction protein occluding (green fluorescence) whereas tissues exposed to acid had very little visible apical epithelial occludin (× 200). However, pretreatment with SPI-8811 prevented the disorganized appearance of occludin in response to acid. Note the specific localization of occludin in each panel (arrows).