Side population cells isolated from KATO III human gastric cancer cell line have cancer stem cell-like characteristics

Figure 2 Soft agar assays and fluorescence for KATO III-green fluorescent protein cells. A: Side population (SP) cells (A-2) were more clonogenic than non-side population (NSP) cells (A-1); B: The assay was repeated 3 times, and the column diagram indicated that there was a statistically significant difference in clonogenic efficiency between SP and NSP cells (^aP < 0.05 vs NSP cells); C: KATO III-green fluorescent protein (GFP) cells expressed strong green fluorescence in regular culture, with no mixed colonies resulting from a single KATO III-GFP cell. C-1 was observed under white light, while C-2 was observed under fluorescent light.