Gene expression profiling and endothelin in acute experimental pancreatitis

Figure 8 Endothelin receptor A and B in dorsal root ganglia (T10-12). A: Double staining of Endothelin receptor A with dual staining for neuronal (NeuN) and glial fibrillary acidic (GFAP) markers from naïve animal; B: Dual staining for Endothelin A and NeuN in a pancreatitic animal; C: NeuN in a pancreatitic animal; D: Endothelin receptor A in a pancreatitic animal. Significant increases in the endothelin (ET)-A are noted in dorsal root ganglia (DRG) neurons of all sizes on day 7 after induction of pancreatitis; E: Dual staining for Endothelin B and GFAP in a naïve animal; F: Dual staining for Endothelin B and GFAP in a pancreatitic animal; G: GFAP in a pancreatitic animal; H: Endothelin receptor B in a pancreatitic animal; Significant increases in the ET-B receptor are noted localized in satellite glia and Schwann cell myelin in thoracic DRG on day 7 after induction of pancreatitis. Alexa Fluor 568, red; Alexa Fluor 488, green (n = 5).