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©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 28, 2012; 18(32): 4323-4334
Published online Aug 28, 2012. doi: 10.3748/wjg.v18.i32.4323
Published online Aug 28, 2012. doi: 10.3748/wjg.v18.i32.4323
Table 2 Primers used in this study
| Genes | Primer sequences 5’-3’ |
| HP1 | CTGGAGAGACTAAGCCCTCC |
| HP2A | AACATTACTGACGCTGATTG |
| Kan AatII F | GATTTAGACGTCTCAGGGCGCAAGGGCT |
| Kan AatIIR | TTCCTTTGACGTCTCAGAAGAACTCGTCAA |
| Kan BglII F (Mut) | AGG GGA TCA AGG TCA GAT CAA GAG A |
| Kan BglII R (Mut) | TCT CTT GAT CTG ACC TTG ATC CCC T |
| MCS BamHI F (Mut) | GTC GAC CGA CCC CAA GCT TCT AGA GGT ACC G |
| MCS BamHI R (Mut) | CGG TAC CTC TAG AAG CTT GGG GTC GGT CGA C |
| Kan BamHI F (pIVET12) | GAT TTA GGA TCC TCA GGG CGC AAG GGC T |
| Kan BamHI R (pIVET12) | TTC CTT TGG ATC CTC AGA AGA ACT CGT CAA |
| CAT | CAA CGG TGG TAT ATC CAG |
| MCAT | GCC ATT GGG ATA TAT CAA CGG TGG TAT ATC C |
| NMCAT | CTC CAT TTT AGC TTC CTT AGC TCC TGA AAA TCT CG |
| CagA F | GATGGCGTGATGTTTGTTGATT |
| CagA R | CGTAGCACCCACACCCTTATC |
| CysS F | TGTTATTCCCCCACCATGAAA |
| CysS R | GCAAGCTCCACGCCAAAG |
| Flhf F | TGTTGCCCTGAAGATTTGAATTT |
| Flhf R | TAGCGCGCGGCTAATTTAG |
| FliA F | AAGCAACAACACCACCATCAAG |
| FliA R | GCTGGGCAAGCGCTCTT |
| FliM F | CGCCAGGTGATGCAAATTT |
| FliM R | AGCGACGATTTGGACCACAT |
| FucT F | TGAATGTGCATGATTTCAACAACT |
| FucT R | GCTTTCCCATCAAGGGTGTTT |
| HsdM F | CTGGGACATTCAAATCAATTATGG |
| HsdM R | CGTCTTGCAAGCGTTTAAGATCT |
| InfB F | GAGTCGCTCACACGGAAGCT |
| InfB R | CAATGAAAGACACCCATTTGTCA |
| LpxC F | CAAGCCCATCATTTTGTCTTTAGTAA |
| LpxC R | CAAGTAATTCACTTCTTGCAAAAACC |
| MinC F | TGCGCAAACACCAGCTTTT |
| MinC R | ATGGCGCTCATAAATCGTTGT |
| MurC F | GGCTCATGGAAAGAGCAGTATCA |
| MurC R | GCCCCAATAATCGCTCCAA |
| MurD F | GCGGCACTAACGGGAAAA |
| MurD R | CCCACTCACAGCCTTAAAATCTTC |
| MurI F | AAAAGCGACGATTCAATCCAA |
| MurI R | AAGTAGCTAAATGCGAAATGTTCAAA |
| Nth F | TGAATTATTGGTGGCGACCAT |
| Nth R | TTGGGCGTTATTTGATTCACTCT |
| Omp26 F | CAAATCGGCACCGTTACCA |
| Omp26 R | CATGAAATCCCGCTAGAAGCA |
| Pgi F | AGCCCACCACAGGGTGTTT |
| Pgi R | CTCCAATATTGCTTGGTGAAATCTT |
| PriA F | GGAGGAGCGCTAGGCAAAT |
| PriA R | AGTTCGCACCTTTCTTGCAATT |
| RbfA F | GCGAGTTGAATTCTTTAAGCGTTAC |
| RbfA R | GACCCTCAGCTTTTTTCAATTTAGA |
| RecG F | GCGATCAACAAAACGCCATTA |
| RecG R | GCACCCCACATCGCCTATAA |
| SodB F | TGCTAAAGACAGCATGGGAGATT |
| SodB R | TTCACATAAGTTTGATGGTGTTTCC |
| TlpC F | TGGTTAGCGCGATTTATACGAA |
| TlpC R | GGGTAGGCGATTTTTGAATCC |
| TnpB F | TTGGTGTTTAGAATGCGGGTATAG |
| TnpB R | TTTCCTACCCCAAAAAGACTTAACC |
| TrxB F | CGCCATTGCTTTGTGCAA |
| TrxB R | TGATGCGGCTGAATTCTTTTT |
| Type II R / M F | TTCTATAACAGCACCGCTGACATT |
| Type II R / M R | CGCGTATATTTGTTAGAAGTGATGAAA |
| VacA F | GGGTTATGCCAGACAAATGATTG |
| VacA R | TCTTATGCTCTAAACTGGCTATGTTGTT |
| VirB4 F | GACCATAGCCCTTATTTGTTTAATTTTC |
| VirB4 R | CTCCTATAATCATGGTATGTCCCACTAC |
| Ycf5 F | AGCGCTATCAATGAAATGAGCAT |
| Ycf5 R | CCACACCCCGCCTAAGAAA |
| HP0423 F | TCGCTCCTTAAGGTTACACGATT |
| HP0423 R | TCAAAGCCACCATCAATAACAAA |
| HP0424 F | CGCTGTTTTTAGTTTTAGAGGCTTTC |
| HP0424 R | GGCTAGGGAAGTGGCTCAAA |
| HP0426 F | TGCGGTATAGGCTTTCATGAACT |
| HP0426 R | AAGGTGTTCAAAGACAGCAAAAAA |
| HP0427 F | TTGCGGTGTGGGTTAATGAA |
| HP0427 R | GCAACGCTACCATACTTTTATCACTT |
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Citation: Singh A, Hodgson N, Yan M, Joo J, Gu L, Sang H, Gregory-Bryson E, Wood WG, Ni Y, Smith K, Jackson SH, Coleman WG. Screening
Helicobacter pylori genes induced during infection of mouse stomachs. World J Gastroenterol 2012; 18(32): 4323-4334 - URL: https://www.wjgnet.com/1007-9327/full/v18/i32/4323.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i32.4323