Gene expression profiling and endothelin in acute experimental pancreatitis

Table 1 Experimental design

No.	Groups (n = 5 per group)	iv injection	Drug administration (ip)
1	Naïve	Non	Non
2	Vehicle/PBS	Vehicle	0.1 mol/L PBS
3	Vehicle/BQ-123 or BQ-788	Vehicle	300 μmol/L
4	DBTC/PBS	DBTC	0.1 mol/L PBS
5	DBTC/BQ-123 or BQ-788	DBTC	33 μmol/L
6	DBTC/BQ-123 or BQ-788	DBTC	100 μmol/L
7	DBTC/BQ-123 or BQ-788	DBTC	300 μmol/L

Rats were injected with dibutyltin dichloride (DBTC) or vehicle (ethanol + glycerol) via tail vein to induce pancreatitis. On day 7 animals were treated with sham or endothelin receptor A (BQ-123) or endothelin receptor B (BQ-788) antagonists and tested for the behavioral hypersensitivity. Each group was constituted of at least n = 5. Experiment was repeated at least once. PBS: Phosphate buffer.