siRNA-mediated downregulation of TC21 sensitizes esophageal cancer cells to cisplatin

Figure 2 TC21 protein and extracellular signal-regulated kinase 2 level. A: Western blotting was carried out using specific antibody; the panel shows inhibition of TC21 protein compared with cells without transfection or transfection with a negative control siRNA (scrambled sequence); B: Bar diagram showing more than 95% decrease in TC21 protein level after TC21 knockdown compared with untreated mock and nonspecific scrambled sequence; C: Western blotting analyses show inhibition of ERK2 protein by targeted siRNA as positive control; D: Bar diagram showing more than 98% decrease in ERK2 level after MAPK knockdown compared with untreated mock and nonspecific scrambled siRNA sequence. ERK: Extracellular signal-regulated kinase; MAPK: Mitogen-activated protein kinase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.