siRNA-mediated downregulation of TC21 sensitizes esophageal cancer cells to cisplatin

Figure 1 Small interfering RNA-targeting TC21/R-Ras2 was transfected in TE13 cells. A: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis: Small interfering RNA (siRNA)-targeting TC21/R-Ras2 was transfected in TE13 cells in a dose-dependent manner. After 48 h and 72 h, cells were lysed, RNA was isolated and the mRNA level was determined semiquantitatively by RT-PCR using TC21-specific primers; B: Bar diagram showing fold change in level of TC21 transcripts after TC21 knockdown.