siRNA-mediated downregulation of TC21 sensitizes esophageal cancer cells to cisplatin

Table 1 Cell cycle analyses of siRNA-treated TE13 cells using flow cytometry

Experimental parameters	SubG0 (%)	G1 (%)	S (%)	G2/M (%)
Untreated TE131 cells for 24 h	5.7	37	21	34.8
Cisplatin treatment (8 μg/mL) for 24 h	17.8	30.6	11.8	38.2
Untreated TE13 cells for 48 h	7.9	34.7	10	41
TC21 siRNA2 (20 nmol) transfection for 48 h	14.2	22.8	12.2	42.3
Untreated TE13 cells for 72 h	10.3	36.7	27.7	25.8
TC21 siRNA transfection (20 nmol) for 48 h followed by cisplatin treatment for 24 h (total time of treatment is 72 h)	38.4	23.8	11.9	12.8

¹Esophageal squamous cell carcinoma cells;

²Small interfering RNA.