Increased expression of chondroitin sulphate proteoglycans in rat hepatocellular carcinoma tissues

Figure 4 Heparan sulphate glycosaminoglycan staining in rat liver tissues. Heparan sulphate (HS) glycosaminoglycan (GAG) content in tissue was stained using 10E4 antibody (dark red). A and D: Control group; B and E: Hepatocellular carcinoma (HCC) tissues with intensive HS GAG staining; C and F: HCC tissues with relative weaker HS GAG staining. Long black arrows: Fibrous tissue septa; short black arrows: The cells are magnified in the small boxes; G: Comparison of the average integrated optical density (IOD) in HS GAG positive staining in liver tissues between the control and HCC model groups (^bP < 0.01). IOD/area: Integrated optical density per stained area.