Orotate phosphoribosyl transferase mRNA expression and the response of cholangiocarcinoma to 5-fluorouracil

Figure 4 Relationship between chemosensitivity to 5-fluorouracil and mRNA expression of thymidine phosphorylase, orotate phosphoribosyl transferase, thymidylate synthase, and dihydropyrimidine dehydrogenase in 23 cholangiocarcinoma tissues. Chemosensitivity to 5-fluorouracil (5-FU) and mRNA expression were analyzed for 23 cholangiocarcinoma (CCA) tissues using histoculture drug response assay and quantitative real-time reverse transcription-polymerase chain reaction. The responders and non-responders were classified using the median value of the % I.I. as the cut-off point. The mRNA expression of orotate phosphoribosyl transferase (OPRT) was significantly higher in the responder (P < 0.05) group. The mRNA levels of thymidylate synthase (TS), and dihydropyrimidine dehydrogenase (DPD) and thymidine phosphorylase (TP) in the non-responder and responder groups, however, were not significantly different. The long, thin lines represent the mean, and the error bars represent the SD values. Each symbol (circles and squares) represents one CCA tissue sample.