Orotate phosphoribosyl transferase mRNA expression and the response of cholangiocarcinoma to 5-fluorouracil

Figure 1 Photomicrographs (40 ×) of hematoxylin and eosin-stained and terminal deoxynucleotide transferase-mediated dUTP nick-end label-stained sections of cholangiocarcinoma tissues cultured on collagen gel sponges for 4 d in the absence or presence of 5-fluorouracil. Tumor cells with anaplastic characteristics showing hyperchromatic nuclei/cytoplasm are indicated with short arrows. Cells with eosinophilic cytoplasm with shrunken (condensed) nuclei (pyknotic nuclei) and fragmented nuclei (karyorrhexis) are indicated with long arrows. 5-FU: 5-fluorouracil; HE: Hematoxylin and eosin; TUNEL: Terminal deoxynucleotide transferase-mediated dUTP nick-end label