Copyright
©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 14, 2012; 18(30): 3977-3991
Published online Aug 14, 2012. doi: 10.3748/wjg.v18.i30.3977
Published online Aug 14, 2012. doi: 10.3748/wjg.v18.i30.3977
Table 1 Sequences of oligonucleotide primers and conditions for real-time polymerase chain reaction
Gene target | Genbank number (mRNA) | Oligonucleotide1 (5'- to 3'-) | Annealing temperature (°C) | Product size (bp) |
Occludin | NM-031329 | F: GCTCAGGGAATATCCACCTATC R: TTCTCCAGCAACCAGCATC | 60 | 344 |
ZO-1 | NM-001106266 | F: CCACAGACATCCAACCAGC R: AGCCCAAAGAACAGAAGACC | 60 | 247 |
Claudin-1 | NM-031699 | F: GCCTCCAATGCCGTTCT R: TGCCTGCGTCCCTCTTG | 60 | 317 |
Claudin-4 | NM-001012022 | F: GTTCCCGCCAGCAACTATG R: CCTTCAGCCCCGTATCCA | 60 | 282 |
JAM-A | NM-053796 | F: CCTCCATCCAAGCCGACA R: CAAAGACCAAATCCCCTGAC | 60 | 211 |
prkC | NM-001105713 | F: GATGGACGGGGTCACGA R: CGCTTGGCAGGGTGTTT | 60 | 165 |
β-actin | NM-031144 | F: CAGGTCATCACTATCGGCAAT R: GAGGTCTTTACGGATGTCAAC | 60 | 144 |
-
Citation: Zhou YK, Qin HL, Zhang M, Shen TY, Chen HQ, Ma YL, Chu ZX, Zhang P, Liu ZH. Effects of
Lactobacillus plantarum on gut barrier function in experimental obstructive jaundice. World J Gastroenterol 2012; 18(30): 3977-3991 - URL: https://www.wjgnet.com/1007-9327/full/v18/i30/3977.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i30.3977