Effects of Lactobacillus plantarum on gut barrier function in experimental obstructive jaundice

Table 1 Sequences of oligonucleotide primers and conditions for real-time polymerase chain reaction

Gene target	Genbank number (mRNA)	Oligonucleotide1 (5'- to 3'-)	Annealing temperature (°C)	Product size (bp)
Occludin	NM-031329	F: GCTCAGGGAATATCCACCTATC R: TTCTCCAGCAACCAGCATC	60	344
ZO-1	NM-001106266	F: CCACAGACATCCAACCAGC R: AGCCCAAAGAACAGAAGACC	60	247
Claudin-1	NM-031699	F: GCCTCCAATGCCGTTCT R: TGCCTGCGTCCCTCTTG	60	317
Claudin-4	NM-001012022	F: GTTCCCGCCAGCAACTATG R: CCTTCAGCCCCGTATCCA	60	282
JAM-A	NM-053796	F: CCTCCATCCAAGCCGACA R: CAAAGACCAAATCCCCTGAC	60	211
prkC	NM-001105713	F: GATGGACGGGGTCACGA R: CGCTTGGCAGGGTGTTT	60	165
β-actin	NM-031144	F: CAGGTCATCACTATCGGCAAT R: GAGGTCTTTACGGATGTCAAC	60	144

¹Primers were designed based on sequences of rat-corresponding genes from the GenBank database. JAM-A: Junction adhesion molecule-A; ZO-1: Zonula occludens-1.