High level of urokinase plasminogen activator contributes to cholangiocarcinoma invasion and metastasis

Figure 3 Levels of urokinase plasminogen activator, plasminogen activator inhibitor type 1 protein and urokinase plasminogen activator receptor expression in two cholangiocarcinoma cell lines and immortalized cholangiocytes. A: Levels of secreted urokinase plasminogen activator (uPA) in 40× concentrated conditioned medium and cell-surface-bound uPA were determined by plasminogen-gelatin zymography; B and D: Levels of secreted uPA (B); and secreted plasminogen activator inhibitor type 1 (PAI-1) (D) proteins were determined by western blotting; C: Level of uPAR mRNA was examined by SYBR Green-based qPCR and the data were calculated as relative uPAR expression compared to that of HuCCA-1, 2^–ΔΔCt, and expressed as mean ± SE from three independent experiments. Significant difference is indicated by ^aP < 0.05.