Comparative quantification of human intestinal bacteria based on cPCR and LDR/LCR

Table 4 Experiment designIand II (detected with probe mixture No. 1 and 2)

Trial	Factor
	Enterobacterium c	Eubacterium	Bifidobacterium	Enterococcus	Lactobacillus casei	Lactobacillus acidophilus	IC 1#	IC 2#
Detected with probe mixture No. 1
1	1	0.5	0.0625	0.01	0.005	0.000625	0.25	0.0025
2	0.5	0.0625	0.125	0.005	0.000625	0.00125	0.0625	0.000625
3	0.25	0.25	0.25	0.0025	0.0025	0.0025	1	0.01
4	0.125	1	0.5	0.00125	0.01	0.005	0.125	0.00125
5	0.0625	0.125	1	0.000625	0.00125	0.1	0.5	0.005
Detected with probe mixture No. 2
1	1	0.005	0.000625	0.01	0.005	0.000625	0.25	0.0025
2	0.5	0.000625	0.00125	0.005	0.000625	0.00125	0.0625	0.000625
3	0.25	0.0025	0.0025	0.0025	0.0025	0.0025	1	0.01
4	0.125	0.01	0.005	0.00125	0.01	0.005	0.125	0.00125
5	0.0625	0.00125	0.01	0.000625	0.00125	0.1	0.5	0.005

“1” is a fixed concentration unit equivalent to 105/μL which was diluted by 104 from standard DNA concentration. IC: internal control.