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©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Jan 21, 2012; 18(3): 268-274
Published online Jan 21, 2012. doi: 10.3748/wjg.v18.i3.268
Published online Jan 21, 2012. doi: 10.3748/wjg.v18.i3.268
Table 2 Specific probes
| Genus | Ligase detection reaction probes | Ligase chain reaction antisense probes | Product length (bp) |
| Enterococcus | F: (T)20-TTTGACCACTCTAGAGATAG | F: (T)10-TTGCCCCCGAAGGGGAAGCT | 80 |
| R: P-AGCTTCCCCTTCGGGGGCAA(T)20-FAM | R: CTATCTCTAGAGTGGTCAAA-(T)10 | ||
| Enterobacterium | F: (T)23-TTGGAGGTTGTGCCCTTGAG | F: CTCAAGGGCACAACCTCCAA-(T)10 | 85 |
| R: P-GCGTGGCTTCCGGAGCTAAC(T)22-FAM | R: (T)10-GTTAGCTCCGGAAGCCACGC | ||
| Eubacterium | F: (T)25-TTGACATATGGGTGAAGCGG | F: CCGCTTCACCCATATGTCAA-(T)10 | 90 |
| R: P-GGGAGACCCCGTGGCCGAGA(T)25-FAM | R: (T)10-TCTCGGCCACGGGGTCTCCC | ||
| Bifidobacterium | F: (T)28-GGATGTGGGGCCCGTTCCA | F: (T)10-TAGCTCCGACACGGAACCCG | 95 |
| R: P-CGGGTTCCGTGTCGGAGCTAT(T)27-FAM | R: TGGAACGGGCCCCACATCCA-(T)10 | ||
| Lactobacillus casei | F: (T)30-CAGGTCTTGACATCTTTTGA | F: (T)10-AAACCTGATCTCTCAGGTGA | 100 |
| R: P-TCACCTGAGAGATCAGGTTT(T)30-FAM | R: TCAAAAGATGTCAAGACCTG-(T)10 | ||
| Lactobacillus acidophilus | F: (T)33-GGTCTTGACATCTAGTGCAA | F: (T)10-GAACTCCGTATCTCTACGGA | 105 |
| R: P-TCCGTAGAGATACGGAGTTC(T)32-FAM | R: TTGCACTAGATGTCAAGACC-(T)10 | ||
| IC 1# | F: (T)30-CACAGGGCTTTCCACCATCCGTGTC | 110 | |
| R: P-GTAGCGGCCAAGCTGCCACGACAGG(T)30-FAM | |||
| IC 2# | F: (T)-33-GACATTCGGCAGGCAATCACAGCCT | F: (T)10-GTAAGGTCTTGCAAACGTTCACATC | 115 |
| R: P-GATGTGAACGTTTGCAAGACCTTAC(T)32-FAM | R: AGGCTGTGATTGCCTGCCGAATGTC-(T)10 |
- Citation: Tang ZR, Li K, Zhou YX, Xiao ZX, Xiao JH, Huang R, Gu GH. Comparative quantification of human intestinal bacteria based on cPCR and LDR/LCR. World J Gastroenterol 2012; 18(3): 268-274
- URL: https://www.wjgnet.com/1007-9327/full/v18/i3/268.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i3.268