Mutual regulation between microRNA-373 and methyl-CpG-binding domain protein 2 in hilar cholangiocarcinoma

Figure 4 Methyl-CpG binding domain proteins expression and enrichment in fragment of promoter-associated CpG island. A: Expression methyl-CpG binding domain proteins (MBPs) in hilar cholangiocarcinoma. Compared to control, 2.9-fold increase of methyl-CpG-binding domain protein (MBD)2 was found while no difference of MBD1 and Mecp2 were detected; B: Chromatin immunoprecipitation (ChIP)-polymerase chain reaction analysis showed selective enrichment of MBD2 at region of CpG island; C: Correlation between MBD2 enrichment and different frequency of CpG island methylation. Remarkable difference was observed between super-/hyper-methylation and stand-/hypo-methylation group (^aP < 0.05, ^bP < 0.01 vs control). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.